| Instrument Specification-Mass spectrometer (Triple Quadruple) |
| Mass range(m/z) | 5-2000 amu |
| Scan speed | >10,000 amu/sec |
| Mass Resolution | Unit Resolution FWHM，0.6-0.8 amu |
| Mass stability | ± 0.1Da in 24 hours |
| Sensitivity MRM mode | ESI+, Reserpine 1 pg on column, MRM609/195，S/N > 200,000 |
| Repeatability | ESI+, Tacrolimus 5pg on column with 10 injections, Peak area RSD <
1.5%, Retention time RSD<0.25 |
| Scan types | Full scan MS and selected ion for both Q1 and Q3, product ion scan, precursor ion scan, neutral loss or gain scan, Multiple reaction
monitoring (MRM) |
| Dynamic range | 6 orders of magnitude |
| Ionization source | Ion Source housing with ESI Probe or APCI probe (max temp: 750ºC) ESI flow rate range: 5 μL/min to 3 mL/min
APCI flow rate range: 200 µL/min to 3 mL/min |
| Software | B&C ITECH Chemstation V1.0 |
| Instrument Specification-Liquid Chromatograph (UHPLC) |
| Flow rate range | 0.001 - 2.00 ml/min, 0.001 ml/min increments |
| Pressure range | 0-1300 bar |
| Flow precision | ≤ 0.075 % RSD or 0.005 min SD, whichever is greater, H2O in a flow
range of 0.200 - 2.000 mL/min |
| Flow accuracy | ± 1% or ± 10 μL/min, whichever is greater, H2O in a flow range of
0.200 - 2.000 mL/min |
Gradient composition
range | 0 - 99% (A in B) |
Gradient composition
accuracy | ± 0.5 % absolute from 5 - 95%, H2O in a flow range of 0.200 - 2.000
mL/min |
Gradient composition
precision | ≤ 0.15% or 0.01 min SD, whichever is greater, H2O in a flow range of
0.200 - 2.000 mL/min |
| Injection modes | Full loop, partial loopfill and µL pickup mode |
| Injection volume | Programmable from 0 uL-100 uL,1 µL increments |
| Reproducibility | RSD £ 0.3% for full loop injections |
