Leica DFC365 FX offers excellent fluorescence documentation and fast time-lapse recordings under low light conditions. Based on the state-of-the-art Sony EXview HAD ICX285 sensor®, this cooled camera is exceptionally well suited for a wide range of fluorescence applications from basic fluorescence imaging to demanding applications such as TIRF, FRET, and Structured Illumination.

 

◇ Highly sensitive 1.4 megapixel Sony EXview HAD ICX285 sensor®.

◇Regulated Peltier cooling for excellent signal-to-noise ratio.

◇Two imaging modes: standard and nIR mode with at least 1.5 times enhanced sensitivity in the near infrared range of the spectrum （Quantum efficiency : 700-1000 nm ）.

Nir mode selection provides significant quantum efficiency enhancement and makes this camera ideal for observation CY7 CY5 fluorescent dye.

◇ Three pixel clocking rates (1.6 MHz, 20 MHz, 40 MHz) for full control of image quality and acquisition speed.

◇ groundbreaking acquisition speed with up to 21 fps in full frame and 122 fps in 8x8 binning mode.

◇ high-speed live cell imaging --- unique in this class of fluorescence cameras is the flexibility of three pixel clocking rates to define readout speed of the sensor. In addition to the 1.6 MHz-mode, the Leica DFC365 FX offers 20 MHz and 40 MHz. The 40 MHz mode in particular is dedicated to fast, real-time time-lapse recording. In combination with an overlapping mode, where the signal is read out while the next image is acquired, the user can easily execute high-speed experiments. With a maximum of 21 frames per second in full frame mode (1392 x 1040) or over 76 fps with 4 x 4 binning, the Leica DFC365 FX is the high-performer in this class of scientific fluorescence CCD cameras.

◇ ultimate cell protection --- Minimizing exposure times during time-lapse recordings is crucial to avoid photo-damaging the specimen. The sensor's high sensitivity and the careful selection of glass interfaces within the camera – in combination with nIR mode –are ideal requisites to protect cells and ensure optimal acquisition conditions during live cell imaging.

 

 

Digital camera Leica DFC365FX Technical Data
camera type	Digital monochrome, high-sensitivity cooled camera for fluorescence microscopy
housing	Aluminum, Size (L × W × H) 132 mm × 74 mm × 71 mm
Sensor	Sony ICX285, EXview HAD CCD technology, 2/3“ interline transfer progressive scan CCD
number of pixels	1392 x 1040
pixel size	6.45 μm × 6.45 μm
sensitive surface	9.00 mm × 6.70 mm
full well capacity	18.000 electrons typical
exposure time	7 μseconds – 10 minutes (depends on software)
cooling	one-stage Peltier cooling
dark noise	6 electrons typical (1.6 MHz, 12 bit)
dark current	< 0.5 electrons/pixel/second
dynamic range	> 69 dB typical (1.6 MHz)
operating modes	Standard mode and nIR mode
a/d converter	14 bit
bit depth	8 bit/12 bit
partial scan	Freely definable RoI (region of interest), combination with binning possible
power supply	12 V via FireWire cable
data	IEEE 1394b FireWire 9-pin single cable
c-mount	0.7 x
analog gain	1× – 10×

 

 

image formats	pixel clock mhz	fps*
full frame （1392*1040）	1.6	1
	20	11
	40	21
2x2 binning (696 x 520)	1.6	2
	20	22
	40	42
3x3 binning (464 x 346)	1.6	3
	20	31
	40	63
4x4 binning (348 x 260)	1.6	4
	20	38
	40	77
8x8 binning	1.6	8
	20	61
	40	122

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